Tissue Quality is an Important Determinant of Telomerase Activity as Measured by TRAP Assay
نویسندگان
چکیده
منابع مشابه
Tissue quality is an important determinant of telomerase activity as measured by TRAP assay.
Telomerase is a ribonucleoprotein with the function of a DNA polymerase, in which a segment of the RNA component operates as an internal template. It adds hexameric (TTAGGG) repeats to the telomeric ends of the chromosomes, thus compensating for the continued erosion of the telomeres that occurs in its absence (3,7). Reactivation of telomerase might be a necessary event for the sustained growth...
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Abstract Background and Objective: Breast cancer is the most common cancer in women. Telomerase enzyme is one of the major factors causing the development and proliferation of immortal cells such as cancer cell. The aim of this study was to evaluate telomerase activity in breast tissues of breast cancer patients and healthy people. Material and Methods: In this descriptive study, the sampl...
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BACKGROUND Recent studies have associated telomerase with prognostic factors and survival in neuroblastoma. PROCEDURE We examined telomerase activity by telomere repeat amplification protocol (TRAP) and expression of the RNA component of telomerase (hTR) by Northern blotting in 106 primary neuroblastoma tumors and 22 established cell lines. RESULTS Overall survival at 5 years for all 106 tu...
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Telomeric repeat amplification protocol (TRAP)--a sensitive, PCR-based assay to detect telomerase activity was quintessential to the evaluation of telomerase role in telomere maintenance, cell proliferation, tumour development, and cell immortalization. The assay, however, suffers from many limitations. The most significant are: lack of telomerase activity quantification, changes of the enzyme ...
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introduction: telomeric repeat amplification protocol assay (trap assay) is a standard established technique to detect the activity (expression) of telomerase in the cancerous samples. it includes a time-consuming procedure that has significant drawbacks such as high cost, complicacy, time-consuming procedure, equipment-dependent steps and false-positive signals caused by inclusion of pcr insid...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98254dt05